Signalling
SPusp45

Part:BBa_K1830002:Design

Designed by: Mathieu Husser   Group: iGEM15_Concordia   (2015-08-06)

SPusp45


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

GCA to GCT mutation to remove PstI cut site


Source

Lactococcus Lactis USP45 protein, GenBank accession number: M60178.1

References

Le Loir, Y., Nouaille, S., Commissaire, J., Bretigny, L., Gruss, A., & Langella, P. (2001). Signal peptide and propeptide optimization for heterologous protein secretion in Lactococcus lactis. Applied and Environmental Microbiology, 67(9), 4119-4127.